Journal: Journal of medicinal chemistry

Article Title: Development of a Redox-Sensitive Spermine Prodrug for the Potential Treatment of Snyder Robinson Syndrome

doi: 10.1021/acs.jmedchem.1c00419

Figure Lengend Snippet: Polyamine levels in wild-type (a: CMS-24949) and SMS mutant (b: CMS-26559, c: CMS-6233, and d: CMS-23916) fibroblast cell lines with or without treatment of Spm or prodrug 1 (5 μM) in the presence of AG (1 mM) after 72 h incubation at 37 °C. Values represent data from the experiment performed in triplicates ± SD, *p < 0.05, *** p < 0.001, **** p < 0.0001.

Article Snippet: The authors wish to thank Dr. Charles Schwartz at the Greenwood Genetic Center for supplying the SRS cell lines and control wt fibroblasts and the Snyder Robinson Foundation for financial support of this work (UCF25068A04 to O.P.).

Techniques: Mutagenesis, Incubation

Journal: Journal of medicinal chemistry

Article Title: Development of a Redox-Sensitive Spermine Prodrug for the Potential Treatment of Snyder Robinson Syndrome

doi: 10.1021/acs.jmedchem.1c00419

Figure Lengend Snippet: Polyamine levels in CMS-23916 (mutant) fibroblast cell lines untreated (UT) or treated with prodrug 1 (5 μM) only, NAC (2 mM) only, or a combination of prodrug 1 (5 μM) and NAC (2 mM). Values represent data from the experiment performed in triplicates ± S.D. *p < 0.05.

Article Snippet: The authors wish to thank Dr. Charles Schwartz at the Greenwood Genetic Center for supplying the SRS cell lines and control wt fibroblasts and the Snyder Robinson Foundation for financial support of this work (UCF25068A04 to O.P.).

Techniques: Mutagenesis

Journal: Journal of Diabetes Research

Article Title: Diabetes Is Associated with Increased Autoreactivity of Mannan-Binding Lectin

doi: 10.1155/2017/6368780

Figure Lengend Snippet: Kidney-to-body weight ratio, albumin-to-creatinine ratio, and kidney glomerulus estimation. (a) Kidney-to-body weight ratio of diabetic C57BL/6J WT mice ( n = 6), nondiabetic control C57BL/6J WT mice ( n = 6), diabetic C57BL/6J MBL/DKO mice ( n = 6), and nondiabetic control C57BL/6J mice MBL/DKO ( n = 6). (b) Albumin-to-creatinine ratio (AcR) measurements at study end for WT and MBL/DKO mice. (c and d) Light microscope representation of glomerulus from diabetic C57BL/6J MBL/DKO mice (c) and nondiabetic C57BL/6J MBL/DKO control mice (d). (e) Estimation of glomerulus volume in diabetic C57BL/6J MBL/DKO mice ( n = 13) and nondiabetic control C57BL/6J MBL/DKO mice ( n = 8). Statistical tests are indicated in figure when significant ( ∗ p < 0.05, ∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001). Data is presented as mean (solid line) with SDs (whiskers).

Article Snippet: The albumin-to-creatinine ratios were significantly elevated in the diabetic mice in both the WT group, 212.7 mg/g (CI: 172.2–253.2), as compared to the nondiabetic control WT mice, 61.2 mg/g (CI: 50.2–72.1) ( p < 0.0001), and the diabetic MBL/DKO group, 150.9 mg/g (CI: 116.1–185.7) as compared to nondiabetic control MBL/DKO mice, 43.9 mg/g (CI: 27.7–60.0) ( p < 0.0001), respectively ( ).

Techniques: Control, Light Microscopy

Journal: Journal of Diabetes Research

Article Title: Diabetes Is Associated with Increased Autoreactivity of Mannan-Binding Lectin

doi: 10.1155/2017/6368780

Figure Lengend Snippet: Blood glucose and weight gain of diabetic and nondiabetic control MBL/DKO and WT C57BL/6J mice. (a and b) Blood glucose and weight measurements for diabetic mice destined to be treated with rMBL AF680 ( n = 6), diabetic mice destined to be treated with PBS ( n = 6), control mice destined to be treated with rMBL AF680 ( n = 6), and control mice destined to be treated with PBS ( n = 6). (c and d). Comparison of the development of diabetes according to rising blood glucose levels and weight gain between diabetic ( n = 6) and control ( n = 6) C57BL/6J MBL/DKO mice and diabetic ( n = 6) and control ( n = 6) C57BL/6J WT mice. Statistical tests are given in the figure when significant, and comparisons are made between STZ treated and untreated groups at study end ( ∗∗∗∗ p < 0.0001). Values are given in mean (dots) with SD (whiskers).

Article Snippet: The albumin-to-creatinine ratios were significantly elevated in the diabetic mice in both the WT group, 212.7 mg/g (CI: 172.2–253.2), as compared to the nondiabetic control WT mice, 61.2 mg/g (CI: 50.2–72.1) ( p < 0.0001), and the diabetic MBL/DKO group, 150.9 mg/g (CI: 116.1–185.7) as compared to nondiabetic control MBL/DKO mice, 43.9 mg/g (CI: 27.7–60.0) ( p < 0.0001), respectively ( ).

Techniques: Control, Comparison

Journal: Journal of Diabetes Research

Article Title: Diabetes Is Associated with Increased Autoreactivity of Mannan-Binding Lectin

doi: 10.1155/2017/6368780

Figure Lengend Snippet: In vivo and ex vivo detection of AF680 labelled rMBL 24 hours postinjection. (a) Fluorescent and spectral unmixing images representing one mouse in each group exhibiting distribution of labelled rMBL 24 hours postinjection (dorsal view). (b) Region of interest (ROI) quantification of detected fluorescent signal from diabetic C57BL/6J MBL/DKO mice + AF680 rMBL ( n = 6), diabetic mice + PBS ( n = 6), nondiabetic mice + AF680 rMBL ( n = 6), and nondiabetic mice + PBS ( n = 6) given in average radiant efficiency. (c) Ex vivo fluorescent and spectral unmixing images representation of the left and right kidneys from one mouse in each group. (d) ROI quantification of detected fluorescence in the right and left kidneys of mice in each group as in (b) with values given in average radiant efficiency. Scale bars are given in radiant efficiency. Statistical tests are indicated in the figure when significant ( ∗∗ p < 0.001, ∗∗∗∗ p < 0.0001). The experiment was repeated five times with comparable results.

Article Snippet: The albumin-to-creatinine ratios were significantly elevated in the diabetic mice in both the WT group, 212.7 mg/g (CI: 172.2–253.2), as compared to the nondiabetic control WT mice, 61.2 mg/g (CI: 50.2–72.1) ( p < 0.0001), and the diabetic MBL/DKO group, 150.9 mg/g (CI: 116.1–185.7) as compared to nondiabetic control MBL/DKO mice, 43.9 mg/g (CI: 27.7–60.0) ( p < 0.0001), respectively ( ).

Techniques: In Vivo, Ex Vivo, Fluorescence

Journal: Journal of Diabetes Research

Article Title: Diabetes Is Associated with Increased Autoreactivity of Mannan-Binding Lectin

doi: 10.1155/2017/6368780

Figure Lengend Snippet: Summarized data and statistical information of in vivo and ex vivo imaging of diabetic and nondiabetic mice.

Article Snippet: The albumin-to-creatinine ratios were significantly elevated in the diabetic mice in both the WT group, 212.7 mg/g (CI: 172.2–253.2), as compared to the nondiabetic control WT mice, 61.2 mg/g (CI: 50.2–72.1) ( p < 0.0001), and the diabetic MBL/DKO group, 150.9 mg/g (CI: 116.1–185.7) as compared to nondiabetic control MBL/DKO mice, 43.9 mg/g (CI: 27.7–60.0) ( p < 0.0001), respectively ( ).

Techniques: In Vivo, Ex Vivo, Imaging, Control